Localization of GFP chimeras in transfected parasites. (A) Localization of PFI1755c:GFP. GFP fluorescence, in live cells, is seen in the erythrocyte cytoplasm indicating that the protein is efficiently exported. (B) Localization of PFI1755c:GFP:SDEL. GFP fluorescence, in live cells, remains within the parasite and shows a characteristic ER distribution. (C) Localization of DSS:PFI1755c:GFP. GFP fluorescence, in live cells, is localized diffusely throughout the cytoplasm of the parasite. Panels show a DIC image, GFP fluorescence, GFP (green) and Hoechst (blue) fluorescence overlaid, and a merge of DIC, GFP (green) and Hoechst (blue) images, as indicated. The scale bar is 4mm. (D) Colocalization of PFI1755c:GFP:SDEL with the ER marker, plasmepsin 5. The GFP fluorescence in PFI1755c:GFP:SDEL expressing parasites was directly visualized and the localization of plasmepsin 5 was determined by indirect immunofluorescence, using an antibody raised against plasmepsin 5 . DNA was stained with Hoechst. In the merged image, GFP fluorescence is in green, plasmepsin 5 staining in red, and regions of co-localization in yellow. The scale bar is 4mm.
Osborne AR, Speicher KD, Tamez PA, Bhattacharjee S, Speicher DW, Haldar K. The host targeting motif in exported Plasmodium proteins is cleaved in the parasite endoplasmic reticulum. Mol Biochem Parasitol. 2010 171:25-31. Copyright Elsevier 2010
Other associated proteins
|PF3D7_0936300||ring-exported protein 3|