The secondary antibodies were conjugated to 60–80 nm gold particles. (A) Parasite-infected RBC probed with anti-PfORC1 antibodies. Three nuclei (N1, N2, and N3) are shown in the frame. Arrowheads indicate the deposition of the gold particles within individual nucleus. M - nuclear membrane and P - parasite membrane. R - RBC. The scale bar is shown at the bottom right. (B) A higher resolution magnified version of N1 from (A). Positions of the gold particle deposition and the nuclear membrane (M) are shown. (C) The pattern of the PfORC1 distribution in an individual nucleus of a parasite-infected RBC (different from A).

Mehra P, Biswas AK, Gupta A, Gourinath S, Chitnis CE, Dhar SK. Expression and characterization of human malaria parasite Plasmodium falciparum origin recognition complex subunit 1. Biochem Biophys Res Commun. 2005 337:955-66. Copyright Elsevier 2010.