Immunofluorescence analysis of PfNAPLin asexual and sexual parasites. Acetone fixed parasites from a synchronous asexual culture reacted with rabbit anti-PfNAPL antibodies (1:400), rhodamine-conjugated anti rabbit IgG antiserum (1:200), and DAPI (0.1mgml−1). Fields A–E are photographed with filters specific for rhodamine and DAPI fluorescences, and combined fluorescent signals are shown in the “merge” column. A: early rings; B: late rings; C: trophozoites/early schizonts; D: schizonts; E: free merozoites. Inserts at the right end in panels A and B contain higher magnification of an individual parasite at that time point. Bar is 5 mm. PfNAPL was expressed in all erythrocytic stages of the parasite and predominantly localized in the cytoplasm in asexual and sexual stages of the parasite.
Chandra BR, Olivieri A, Silvestrini F, Alano P, Sharma A. Biochemical characterization of the two nucleosome assembly proteins from Plasmodium falciparum. Mol Biochem Parasitol. 2005 142:237-47. Copyright Elsevier 2009.