Localisation of Pfj4 in infected erythrocytes by immunoEM. (A) Trophozoite-infected erythrocyte. (B) Schizont-infected erythrocyte. Pfj4 localisation was visualised using gold-conjugated anti-rabbit secondary antibodies. Pfj4 was detected in the parasite nucleus and cytoplasm. Pfj4 localisation was also observed in erythrocyte cytoplasm. In some cases, Pfj4 seemed to be associated with membranous structures resembling Maurer’s clefts. The nucleus (n) and the erythrocyte (e) are indicated. Arrows indicate immunostaining in the erythrocyte. Arrowheads indicate immunostaining in the parasite. Size bars, 200 nm.
Pesce ER, Acharya P, Tatu U, Nicoll WS, Shonhai A, Hoppe HC, Blatch GL. The Plasmodium falciparum heat shock protein 40, Pfj4, associates with heat shock protein 70 and shows similar heat induction and localisation patterns. Int J Biochem Cell Biol. 2008 40:2914-26. Copyright Elsevier 2011.