Upper row: PfGAP45 co-localizes with the IMC marker PfMTIP. Immunofluorescence assays were performed using anti-GFP (red) and anti-PfMTIP (green) antibodies on parasites over-expressing PfGAP45-GFP. PfGAP45 shows co-localization with PfMTIP around the periphery of merozoites in mature schizonts.

Middle row: PfGAP45 phosphorylation mutants co-localize with endogenous PfGAP45. Immunofluorescence assays were performed using anti-GFP (green) and anti-PfGAP45 (red) antibodies to localize episomally expressing GAP45-GFP and endogenous PfGAP45.

Lower row: Live Imaging of PfGAP45 GFP in parasites over-expressing PfGAP45-GFP was performed after labeling the nuclei with DAPI.

Thomas DC, Ahmed A, Gilberger TW, Sharma P. Regulation of Plasmodium falciparum Glideosome Associated Protein 45 (PfGAP45) Phosphorylation. PLoS One. 2012;7(4):e35855.