Indirect Immunofluorescence Assay (IFA) was performed using affinity purified anti-PfK1 F4 antibodies. Representative IFAs are shown. Anti-PfK1 IFAs showed no labeling in rings (data not shown) or uninfected RBCs, and increasing RBC membrane labeling in trophozoites and schizonts. The level of labeling increased with parasite maturity and was located at the plasma membrane of the iRBC, in a semi-punctate pattern.
Waller KL, McBride SM, Kim K, McDonald TV. Characterization of two putative potassium channels in Plasmodium falciparum. Malar J. 2008 Jan 24;7:19.