Localisation of PfMRP in P. falciparum schizonts from strain A4. (A) Confocal image of schizont-infected erythrocytes stained using the anti-PfMRP antibody and FITC-labelled secondary antibody. (B) Transmitted light image of the same field. (C) Confocal image of the DAPI-stained schizont nuclei in the same field. Images were captured using a Leica TCS 4D confocal microscope. Arrows in (B) indicate: 1, uninfected human erythrocyte; 2, parasite food vacuole; 3, unstained plasma membrane of an infected human erythrocyte; and 4, stained, disrupted membrane of a presumably formerly infected erythrocyte. Schizonts show a very distinctive pattern of fluorescence staining with anti-PfMRP with the majority of reactivity surrounding what seems to be the plasma membrane of the parasite.
Klokouzas A, Tiffert T, van Schalkwyk D, Wu CP, van Veen HW, Barrand MA, Hladky SB. Plasmodium falciparum expresses a multidrug resistance-associated protein. Biochem Biophys Res Commun. 2004 321:197-201. Copyright Elsevier 2009.