Expression of DsRED and GFP in Plasmodium falciparum double transfectants. Localization of fluorescent proteins DsRED (red) and GFP (green) in pSSPF2/PfHsp60-DsRED–pSSPF2/PfACP-GFP double transfectant (A), pSSPF2/PfACP-DsRED–pSSPF2/PfHsp60-GFP double transfectant (B) and pSSPF2/PfCpn60-DsRED–pSSPF2/PfACP-GFP double transfectants (C–E) was monitored by fluorescence microscopy. +DiC, overlay on a phase contrast image with the blue signal of Hoechst 33342 indicating the position of the nucleus of the parasites. (A, B) Schizont; (C) two co-infecting ring stage parasites; (D) trophozoite; (E) schizont. Scale bar: 5 mm.

Sato S, Wilson RJ. The use of DsRED in single- and dual-color fluorescence labeling of mitochondrial and plastid organelles in Plasmodium falciparum. Mol Biochem Parasitol. 2004 134:175-9. Copyright Elsevier