Localization of components of the mitochondrial branched chain KADH complexes. The localization of the E1β-subunit of P. falciparum BCKDH complex and the mitochondrial lipoamide dehydrogenase were analysed by expressing the first 390 and 653 nucleotides respectively tagged with GFP in erythrocytic stages of the parasite. (A) E1β-subunit of BCKDH. Phase, phase contrast image; GFP–E1β, image obtained with GFP channel; Mito, image obtained with rhodamine channel representing the localization of MitoTracker CMred; Merge, merged image. (B) Mitochondrial lipoamide dehydrogenase. Phase, phase-contrast image; GFP–mE3, image obtained with GFP channel; Mito, image obtained with rhodamine channel representing the localization of MitoTracker CMred; Merge, merged image.

Günther S, McMillan PJ, Wallace LJ, Müller S. Plasmodium falciparum possesses organelle-specific alpha-keto acid dehydrogenase complexes and lipoylation pathways. Biochem Soc Trans. 2005 33:977-80.