PF3D7_1235600 serine hydroxymethyltransferase

Positive control images using endogenously expressed DsRED-tagged ACP instead of anti-ACP antibodies. The use of only one primary antibody, anti-PfSHMTc (C-terminal end), with expressed DsRED tagged Pf ACP, was aimed at eliminating any possibility of artifactual fluorescence arising from interactions between two primary antibodies used simultaneously. (A) Two parasites, upper parasite is undergoing its first division, lower parasite is a late trophozoite. (B) Mitotic schizont with elongating apicoplast. (C) Mitotic schizont with ramifying apicoplast. All parasites show colocalization of anti-PfSHMTc fluorescence with the apicoplast, closely following the shape of the organelle, identical results to those obtained using two primary antibodies (scale bars (A) and (C) 3 μm, (B) 2 μm).

Read M, Muller IB, Mitchell SL, Sims PF, Hyde JE. Dynamic subcellular localization of isoforms of the folate pathway enzyme serine hydroxymethyltransferase (SHMT) through the erythrocytic cycle of Plasmodium falciparum. Malar J. 2010 9:351.