PF3D7_1235600 serine hydroxymethyltransferase

Upper panel: PfSHMTm (N-terminal) apicoplast immunofluorescent images illustrating the concentration of fluorescence in the extremities of elongating apicoplasts. (A) Mitotic schizont with an elongating apicoplast.

Lower panel: GFP-tagging of truncated PfSHMTm in transfected 3D7 parasites. Fluorescence images of parasites transfected to yield a GFP-fusion carrying the first 100 amino acids of PfSHMTm at the N-terminus. MitoTracker was also used to localize the mitochondrion, which showed complete coincidence with the GFP fluorescence. YOYO- stains nuclei.

Read M, Muller IB, Mitchell SL, Sims PF, Hyde JE. Dynamic subcellular localization of isoforms of the folate pathway enzyme serine hydroxymethyltransferase (SHMT) through the erythrocytic cycle of Plasmodium falciparum. Malar J. 2010 9:351.