Upper panel: IFA visualization of subcellular actin in rings using an anti-Pfactin-I antibody (rabbit #2). Second panel: Two-color IFA assay using anti-Pfactin-I (rabbit #2, green) and anti-nuclear pore (PF14_0706) antibody (rat, red). Third panel: Combined FISH/IFA assay in rings showing partial colocalization of chromosome internal var genes (upsC-type) and actin. Fourth panel: IFA analysis of HA-actin-transfected ring stage parasites using mouse anti-HA antibody (red) and anti-Pfactin-I antibody (rabbit #2, green). IFA analysis with anti-Pfactin-I antibodies or with anti-HA antibody in HAactin-transfected 3D7 parasites confirmed the restricted location of actin to the nucleus in ring stage parasites and demonstrated that it concentrated in foci at the nuclear periphery. Colocalization with a nuclear pore antibody indicates that the staining is limited to the inner side of the nucleus.
Zhang Q, Huang Y, Zhang Y, Fang X, Claes A, Duchateau M, Namane A, Lopez-Rubio JJ, Pan W, Scherf A. A critical role of perinuclear ilamentous actin in spatial repositioning and mutually exclusive expression of virulence genes in malaria parasites. Cell Host Microbe. 2011 10:451-63.
Other associated proteins
|PF3D7_1473700||nucleoporin NUP116/NSP116, putative|