Upper panel: Physical clustering of P. falciparum subtelomeric regions detected in trophozoites detected by FISH. The first column of panels shows Nomarski images of parasites, the second shows fluorescent images after DAPI staining (blue), and the third shows FISH signals (yellow) from the single copy Pf11-1 gene probe or the P. falciparum telomere associated rep20 probe. The FISH and DAPI signals are superimposed in the fourth column.
Lower panel: Promiscuous bouquet formation of P. falciparum chromosome ends. Colocalization FISH analysis of heterologous telomeres revealed with Pf11-1-specific (green) and Pf332-specific (red) gene probes. A typical example of an asexual (as) and gametocyte (g) of HB3 parasites is shown for each category.
Freitas-Junior LH, Bottius E, Pirrit LA, Deitsch KW, Scheidig C, Guinet F, Nehrbass U, Wellems TE, Scherf A. Frequent ectopic recombination of virulence factor genes in telomeric chromosome clusters of P. falciparum. Nature. 2000 407(6807):1018-10122.
Other associated proteins