The non-coding telomeric and subtelomeric RNAs colocalize at the nuclear periphery. (A) Telomere, TARE-3 and TARE-6 transcripts were detected by using RNA-FISH assays in parasites infected in the early-schizont (30 h) and late-schizont (36 h) stage. (Top) Early-schizont stage. First row: antisense TARE-6 ss ncRNA was labeled with biotin-16-UTP (red), and antisense Telomere ss ncRNA was labeled with Alexa Fluor 488-5-UTP (green). Second row: Antisense TARE-6 ss ncRNA was labeled with biotin-16-UTP (red), and antisense TARE-3 ncRNA was labeled with Alexa Fluor 488-5-UTP (green). Third row: antisense Telomere ss ncRNA was labeled with biotin-6-UTP (red), and antisense TARE-3 ss ncRNA was labeled with Alexa Fluor 488-5-UTP (green). DAPI staining is shown in blue. (Bottom) Late-schizont stage. First row: Antisense TARE-6. ss ncRNA was labeled with biotin-16-UTP (red), and antisense Telomere ss ncRNA was labeled with Alexa Fluor 488-5-UTP (green). Second row: antisense Telomere ss ncRNA was labeled with biotin-16-UTP (red), and antisense TARE-3 ss ncRNA was labeled with Alexa Fluor 488-5-UTP (green). DAPI staining is shown in blue. In each case, the merged images (yellow) show co-localization of the three small ncRNAs at the nuclear periphery. (B) ncRNA telomeric, TARE-3 and TARE-6 transcripts were detected using RNA-FISH assays in parasites infected in the ring stage. In all the case, the merged images (yellow) show co-localization of the three small ncRNAs at the nuclear periphery and only one signal was observed. Foci were located at the nuclear periphery. In the ring stage, a single perinuclear focus was observed.

Sierra-Miranda M, Delgadillo DM, Mancio-Silva L, Vargas M, Villegas-Sepulveda N, Martínez-Calvillo S, Scherf A, Hernandez-Rivas R. Two long non-coding RNAs generated from subtelomeric regions accumulate in a novel perinuclear compartment in Plasmodium falciparum. Mol Biochem Parasitol. 2012 185(1):36-47. Copyright Elsevier.