The ncRNAs define a novel subdomain in the P. falciparum nucleus. (A) RNA-FISH signals are shown in red, and DNA-FISH signals are shown in green (TARE-6). The signals of the TARE-6 ncRNA and telomeric DNA do not co-localize in ring stages, but in the late-schizont stage some telomere clusters are transcribed and co-localize with TARE-6 ncRNA probe. (B) P. falciparum nucleus present a complex nuclear architecture with several subcompartments localized in the nuclear periphery as are: telomeric clusters, var gene expression site, nucleolus and non-coding RNA compartment. (C) RNA-FISH signals are shown in red (TARE-6), and 28S rDNA DNA-FISH signals are in green. The signals from 28S rDNA and the non-coding TARE-6 transcript do not co-localize. (D) Two-color RNA-FISH of a var2CSA RNA probe (green) and TARE-6 ss ncRNA transcripts (red) for ring stage parasites (FCR3-CSA parasite population). The signals from the var2CSA transcripts and the TARE-6 ncRNAs do not co-localize. In all images, nuclear DNA was stained with DAPI (blue). Scale bars: 1 mm for the ring and schizont stages.
Sierra-Miranda M, Delgadillo DM, Mancio-Silva L, Vargas M, Villegas-Sepulveda N, Martínez-Calvillo S, Scherf A, Hernandez-Rivas R. Two long non-coding RNAs generated from subtelomeric regions accumulate in a novel perinuclear compartment in Plasmodium falciparum. Mol Biochem Parasitol. 2012 185(1):36-47. Copyright Elsevier.
Other associated proteins