Fluorescence in situ hybridization coupled immunofluorescence assay (FISH–IFA). (FISH–IFA was performed using fluorescence analog labeled TARE-6 DNA probe and antibodies against GFP in ORC1N1–238–GFP expressing parasites. The results indicate that majority of the TARE-6 and ORC1N1–238–GFP signals are overlapping or closely associated with each other. Top panel corresponds to ring stage parasite and bottom two panels correspond to trophozoite stage parasites.

Deshmukh AS, Srivastava S, Herrmann S, Gupta A, Mitra P, Gilberger TW, Dhar SK. The role of N-terminus of Plasmodium falciparum ORC1 in telomeric localization and var gene silencing. Nucleic Acids Res. 2012 40(12):5313-31