PF3D7_1028700 merozoite TRAP-like protein

Schizont rich culture was thin smeared on a glass slide, fixed in methanol, stainined with rabbit anti-MTRAP polyclonal serum (1:200) and secondary Alexa-Flor488 anti-Rabbit. Dual colour fluorescence images were captured using a Carl Zeiss Axioskop 2 microscope at 100x using Oil imersion. Nucleus is marked with DAPI. Scale bar indicate 1┬ÁM. Free merozoite is shown with MTRAP clearly marking the apical end (shown to be micronemes by immuno-EM).

Baum J, Richard D, Healer J, Rug M, Krnajski Z, Gilberger TW, Green JL, Holder AA, Cowman AF. A conserved molecular motor drives cell invasion and gliding motility across malaria life cycle stages and other apicomplexan parasites. J Biol Chem. 2006 281(8):5197-208.