Detection of fibrillar structures in Plasmodium falciparum merozoites by immunofluorescence assay. Co-immunostaining of free merozoites with anti-MSP3 Abs along with Abs against EBA-175, AMA-1, Rh2b and MSP5. In all the slides long fibrous structure attached to free merozoite was detected by anti-MSP3 Abs. Monoclonal MSP3 antibodies also stained these fibrillar structures associated with merozoites, but other marker protein did not detect fibrillar assemblies. The long fibrils like structures attached at one end with merozoite surface were consistently seen to associate to distant erythrocytes as well as wrap around them.
Imam M, Singh S, Kaushik NK, Chauhan VS. Plasmodium falciparum Merozoite Surface Protein 3: oligomerization, self-assembly and heme complex formation. J Biol Chem. 2013 289(7):3856-68
Other associated proteins
|PF3D7_0731500||erythrocyte binding antigen-175|
|PF3D7_1035400||merozoite surface protein 3|
|PF3D7_1335300||reticulocyte binding protein 2 homologue b, ALKBH5|