PfRab6 but not PfRab7 or retromer rapidly redistributes to the ER upon brefeldin A treatment. (A) Distributions of DDmCherry-PfRab6, DD-mCherry-PfRab7 and PfVps35-YFP in live parasites after one hour in the presence of 5 mg/mL brefeldin A. Redistribution of DDmCherry-PfRab6 to the perinuclear ER is indicated with an arrow (top panel). FP, fluorescent protein. mCherry fluorescence is pseudocolored red, YFP fluorescence is pseudocolored green and Hoechst 33342 fluorescence is pseudocolored cyan. Scale bar, 2 mm. Golgi marker DD-mCherry-PfRab6 redistributed to the perinuclear ER. Association with the perinuclear ER was not observed for either Rab7 or Vps35. The contrasting responses of the Golgi apparatus and endosome to brefeldin A treatment reveal differences in the dynamics of vesicular traffic to and from these compartments.

Krai P, Dalal S, Klemba M. Evidence for a Golgi-to-Endosome Protein Sorting Pathway in Plasmodium falciparum. PLoS One. 2014 9(2):e89771.