Expression and localization of PfPTPA-GFP (d) and PfPP2A-GFP (e) throughout the erythrocytic cell cycle of P. falciparum were analyzed by fluorescence microscopy after transfection. Examination of live parasites transfected with pfptpa-gfp showed that the signal was confined within the parasite where the distribution is nucleo-cytoplasmic in rings, trophozoites and schizonts (d), as the fluorescence partially overlapped DNA staining. Parasites transfected with the construct containing pfpp2a-gfp showed a nucleo-cytoplasmic distribution in rings and schizonts with an intense accumulation in the nucleus of late trophozoites.

Vandomme A, Fréville A, Cailliau K, Kalamou H, Bodart JF, Khalife J, Pierrot C. PhosphoTyrosyl Phosphatase Activator of Plasmodium falciparum: Identification of Its Residues Involved in Binding to and Activation of PP2A. Int J Mol Sci. 2014 15(2):2431-53.