PPLP2 localization in asexual blood stage parasites. PPLP2-positive vesicular structures are present in the cytoplasm of trophozoites (TZ) and schizonts (SZ). PPLP2 was immunolabelled with mouse anti-PPLP2RP1 antisera (green), the asexual blood stages were visualized by rabbit anti-MSP1 antisera (red). Nuclei were highlighted by Hoechst stain (blue). PPLP2 first appeared in the trophozoites stage and here localized in vesicular structures. Multiple PPLP2-positive vesicular structures were detected also in mature schizonts. The blood stage parasites were highlighted by labelling of MSP1.

Wirth CC, Glushakova S, Scheuermayer M, Repnik U, Garg S, Schaack D, Kachman MM, Weißbach T, Zimmerberg J, Dandekar T, Griffiths G, Chitnis CE, Singh S, Fischer R, Pradel G. Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes. Cell Microbiol. 2014 Mar 7.