(C) Immunofluorescence analysis of red blood cells infected with parental 3D7 parasites co-labelled with a-FIKK4.2 and a-PFB0090c (J-dots) antibodies. Merge and phase overlay images indicate that FIKK4.2 and J-dots do not co-localise in infected red blood cells. In all panels, parasite nuclei are counterstained with DAPI. (D) Immunofluorescence analysis of red blood cells infected with either parental 3D7 or FIKK4.2 knockout (1E2) parasites. Infected red blood cells were incubated with antibodies raised against the knob-associated histidine-rich protein (KAHRP), mature-parasite-infected erythrocyte surface antigen (MESA) or P. falciparum antigen 332 (Pf332). Parasite nuclei were counterstained with DAPI. KAHRP, MESA and Pf332 as three examples of other very well described P. falciparum-encoded exported proteins.
Kats LM, Fernandez KM, Glenister FK, Herrmann S, Buckingham DW, Siddiqui G, Sharma L, Bamert R, Lucet I, Guillotte M, Mercereau-Puijalon O, Cooke BM. An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells. Int J Parasitol. 2014 Feb 14. [Epub ahead of print]
Other associated proteins
|PF3D7_0201800||knob associated heat shock protein 40|
|PF3D7_0202000||knob-associated histidine-rich protein|
|PF3D7_0424700||serine/threonine protein kinase, FIKK family|
|PF3D7_0500800||erythrocyte membrane protein 2 mature parasite-infected erythrocyte surface antigen|