Intracellular distribution of GBP in the presence of BFA Ring-infected erythrocytes were cultured in the presence (a-c) or absence (d) of BFA, fixed and incubated with antibodies to GBP, followed by a mixture of fluorescein isothiocyanate-conjugated secondary antibody and propidium iodite. The same field was analysed for staining of the nuclei (a) or the presence of GBP (b). (e) Superimposition of (a) and (b). (d) Superimposition of nuclear and protein staining in control cells. The arrows highlight the location of GBP. In untreated cells GBP is found in the host cell cytoplasm. In the presence of BFA, GBP was not transported into the erythrocyte, but remained inside the parasite cell.
Benting J, Mattei D, Lingelbach K. Brefeldin A inhibits transport of the glycophorin-binding protein from Plasmodium falciparum into the host erythrocyte. Biochem J. 1994 300:821-6.