(E) Localisation of GRASP2-GFP in unfixed parasites. Free merozoites display GFP fluorescence in one tightly defined compartment (a). A duplication of this compartment takes place in ring-stage parasites prior to nuclear division (b). As the parasite matures from trophozoite (c) to schizont (d) the organelle further multiplies until one compartment can be equally distributed among the forming progeny. (F) Colocalisation of the two GRASP proteins in the double transgenic parasite line. Immunofluorescence assay of GRASP2-GFP (green) with HA-specific antibodies representing GRASP1 (red). The merged image shows colocalisation of the two compartments. One single GRASP2-GFP compartment in close proximity to the nucleus was observed. in free merozoites (Ea). Duplication of the GRASP2-defined compartment occured prior to nuclear division (Eb) resulting in a multiplicity of Golgi compartments in schizonts (Ec,d).

Struck NS, Herrmann S, Langer C, Krueger A, Foth BJ, Engelberg K, Cabrera AL, Haase S, Treeck M, Marti M, Cowman AF, Spielmann T, Gilberger TW. Plasmodium falciparum possesses two GRASP proteins that are differentially targeted to the Golgi complex via a higher- and lower-eukaryote-like mechanism. J Cell Sci. 2008 121(Pt 13):2123-9.