Confocal fluorescence microscopy images of transfected 3D7 P. falciparum-infected RBCs expressing a GFP chimera directed to the red blood cell membrane skeleton. DIC image, the GFP fluorescence signal and an overlay of a Knob-Associated Histidine-Rich Protein 1-123-GFP transfectant Scale bar = 5 µm. The top row shows an early trophozoite stage parasite with KAHRP present in the endomembrane system of the parasite and in the red blood cell cytoplasm. The punctate fluorescent signal in the infected red blood cell (middle row) demonstrates a brief association of the chimera with the Maurer’s clefts. The bottom row illustrates the association of KAHRP with the cytoplasmic face of red blood cell membrane.
Tilley L, McFadden G, Cowman A, Klonis N. Illuminating Plasmodium falciparum-infected red blood cells. Trends Parasitol. 2007 23:268-77.