Immunofluorescence microscopy of fixed cells using anti-PfSec22 antibodies and goat anti-rabbit Alexa Fluor 555 secondary antibodies. An intense ring of PfSec22 fluorescence is visible in ring- and trophozoite-infected cells. Isolated foci of PfSec22 fluorescence (arrow) are also detected in the host cell compartment in trophozoite-infected cells, suggesting export of PfSec22 into the erythrocyte cytosol. Scale bars, 2 mm.

Bottom row: Coimmunolocalization and membrane association of endogenous PfSec22. The association of PfSec22 with the parasite ER was investigated by coimmuno-fluorescence assays using rabbit anti-PfSec22 and rat anti-PfBip antibodies, as indicated. Both proteins colocalized significantly within the parasite. Scale bars, 2 mm.

Ayong L, Raghavan A, Schneider TG, Taraschi TF, Fidock DA, Chakrabarti D. The Longin domain regulates the steady-state dynamics of Sec22 in P. falciparum. Eukaryot Cell. 2009 8(9):1330-40.