Apicoplast development and DNA replication are hardly inhibited by calcium ionophores. Fluorescence microscopy done on ACP-GFP expressing parasites indicates that ACP-GFP fusion protein (≈40 kDa) directed towards apicoplast has been imported and processed without much interference even after 6 hours post treatment.

Cheemadan S, Ramadoss R, Bozdech Z. Role of Calcium Signaling in the Transcriptional Regulation of the Apicoplast Genome of Plasmodium falciparum. Biomed Res Int. 2014;2014:869401.