PF3D7_0615100 enoyl-acyl carrier reductase

Localization ENR transit peptide double mutants to apicoplast. A field of Plasmodium cells transiently transfected with indicated ENR mutant. TufA was visualized by a rabbit anti-TufA antibody in conjugation with anti-rabbit Alexa 568 antibody (second panel) while the indicated ENR mutant was expressed in fusion with GFP (third panel). DNA was stained with DAPI (fourth panel), while the first panel displays the phase contrast image of the selected field. The fifth panel is a merge of images obtained from phase contrast, Alexa 568, GFP, and DAPI. (A) GFP-tagged ENR R40E-K42E mutant, (B) GFP-tagged ENR K42E-K49E mutant was localized to the apicoplast along with TufA, (C) GFP-tagged ENR K42A-K44A, (D) GFPtagged ENR K44A-R48A mutant, and (E) GFP-tagged ENR K51A-R52A mutant was diffusely distributed in the cytoplasm

Banerjee T, Singh RR, Gupta S, Surolia A, Surolia N. 15-Deoxyspergualin hinders physical interaction between basic residues of transit peptide in PfENR and Hsp70-1. IUBMB Life. 2012 64(1):99-107.

Other associated proteins

PFID Formal Annotation
PFC10_API0028 elongation factor (tufA)
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