PF3D7_0615100 enoyl-acyl carrier reductase

Basic residues of transit peptide essential for localization ENR to apicoplast. A field of Plasmodium cells transiently transfected with indicated PfENR mutant. TufA (encoded by the apicoplast genome) was visualized by a rabbit anti-TufA antibody in conjugation with anti-rabbit Alexa 568 antibody (second panel) while the indicated ENR mutant was expressed in fusion with GFP (third panel). DNA was stained with DAPI (fourth panel) while the first panel displays the phase constrast image of the selected field. The fifth panel is a merge of images obtained from phase contrast, Alexa 568, GFP, and DAPI. (A) GFP-tagged ENR K49A mutant was localized tothe apicoplast along with TufA, (B) GFP-tagged ENR K51A mutant was mislocalized to the cytoplasm, (C) GFP-tagged ENR R52A mutant was mislocalized to the cytoplasm, (D) GFP-tagged ENR R40A mutant was localized to the apicoplast along with TufA.

(E) GFP-tagged ENR K42A mutant was localized to the apicoplast along with TufA, (F) GFP-tagged ENR K44A mutant was mislocalized to the cytoplasm

Banerjee T, Singh RR, Gupta S, Surolia A, Surolia N. 15-Deoxyspergualin hinders physical interaction between basic residues of transit peptide in PfENR and Hsp70-1. IUBMB Life. 2012 64(1):99-107.

Other associated proteins

PFID Formal Annotation
PFC10_API0028 elongation factor (tufA)
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