Analysis of STEVOR Expression in the 3D7DMAHRP1 Parasites -parasite line in which the disruption of the mahrp1 gene has been shown to compromise trafficking of PfEMP1 to the iRBC surface allowing any adhesion or binding phenotype to be attributed to parasite ligands other than PfEMP1. Live IFA with anti-S1 and anti-S2 (anti-STEVOR Abs) showed that trafficking of STEVOR to the 3D7DMAHRP1-iRBC surface was not impaired. (A) Detection of STEVOR on live 3D7DMAHRP1-R+ -iRBCs surface by anti-S1 and anti-S2 (top panel). Dual staining of live 3D7DMAHRP1-R+ -iRBCs with anti-S2 and anti-GPC antibodies (bottom panel, top row) or anti-SBP1 (bottom panel, middle row) illustrating the external location of both STEVOR and GPC (Glycophorin C). SBP1 internal location revealed by IFA on fixed parasites (bottom panel, bottom row). Only surface-expressed STEVOR was detected by live IFA of iRBCs, as staining with antibody against the internally located Maurer’s clefts protein SBP1 was negative while dual IFA on fixed parasites showed double labeling of STEVOR and SBP1(B) Live wet-preparation images showing successful rosetting enrichment of 3D7DMAHRP1 line.
Niang M, Bei AK, Madnani KG, Pelly S, Dankwa S, Kanjee U, Gunalan K, Amaladoss A, Yeo KP, Bob NS, Malleret B, Duraisingh MT, Preiser PR. STEVOR Is a Plasmodium falciparum Erythrocyte Binding Protein that Mediates Merozoite Invasion and Rosetting. Cell Host Microbe. 2014 16(1):81-93. PMID: 25011110
Other associated proteins