PF3D7_1370300 membrane associated histidine-rich protein

C: Live cell imaging of 3D7 parasites expressing SEMP1 with C-terminal tagged GFP. D: Immunofluorescence assays of MeOHfixed RBCs infected with 3D7 expressing SEMP1 with a C-terminal 3xHA tag, co-labelled with rat a-HA and either rabbit a-MAHRP1 or rabbit a-REX1 antibodies. D: Scatter plot of co-localization of SEMP1 and REX1 in SEMP1-3xHA parasites. E: Electron microscopy (EM) of RBCs infected with 3D7 expressing SEMP1 with a C-terminal GFP tag labelled with rabbit a-GFP antibodies and decorated with 5 nm gold conjugated Protein A. GFP-tagged protein was exported (c). SEMP1-3xHA co-localized with MAHRP1 and REX1 to the MCs. Electron microscopy using antibodies against GFP confirmed the localization of SEMP1-GFP at the MCs (F).

Dietz O, Rusch S, Brand F, Mundwiler-Pachlatko E, Gaida A, Voss T, Beck HP. Characterization of the Small Exported Plasmodium falciparum Membrane Protein SEMP1. PLoS One. 2014 9(7):e103272.

Other associated proteins

PFID Formal Annotation
PF3D7_0702400 small exported membrane protein 1
PF3D7_0935900 ring-exported protein 1