HSP101 is required for activation of PSAC but not trafficking of CLAG3 to the RBC periphery. CLAG3 proteins localize to the infected RBCperiphery and are the only parasite proteins known to be involved in PSAC activation IFA of ring-stage 13F10 parasites showing CLAG3 localization to the RBC membrane with or without TMP (DDD-stabilizing small molecule trimethoprim). TMP was removed in the late schizont stage and parasites were allowed to develop 18 h before fixation with 90% acetone/10% methanol. Scale bar, 5 mm.

Beck JR, Muralidharan V, Oksman A, Goldberg DE. PTEX component HSP101 mediates export of diverse malaria effectors into host erythrocytes. Nature. 2014 Jul 16. [Epub ahead of print]