Subcellular localization of PfPRL. Micrographs represent the co-staining profiles of PfPRL with the Golgi marker PfErd2 (A), the ER marker PfBip (B), and the rhoptry/micronemal protein PfAMA-1 (C). Synchronous 3D7 parasite cultures were probed with purified rabbit anti-PfPRL in combination with antibodies to the compartmental markers. Binding of primary antibodies was detected using Alexa-Fluor 488 conjugated antibodies (organellar markers) and Alexa-Fluor 555-conjugated anti-rabbit IgG (PfPRL). Panel I is differential interference contrast image, panel II is fluorescence due to Alexa-Fluor 488 (green), panel III fluorescence due to Alexa-Fluor 555 (red), while panel IV is a merge of the first three panels. Yellow spots indicate overlap between PfPRL and the respective organellar marker, while blue arrows indicate food PfPRL-associated sites in the digestive vacuole.
Pendyala PR, Ayong L, Eatrides J, Schreiber M, Pham C, Chakrabarti R, Fidock DA, Allen CM, Chakrabarti D. Characterization of a PRL protein tyrosine phosphatase from Plasmodium falciparum. Mol Biochem Parasitol. 2008 158(1):1-10.
Other associated proteins
|PF3D7_1113100||protein tyrosine phosphatase|
|PF3D7_1133400||apical membrane antigen 1|
|PF3D7_1353600||ER lumen protein retaining receptor|