Immunofluorescence assay on CS2 (upper panels) versus CS2ΔPTP1 parasites (lower panels). Immunofluorescence assay probed with a-PfEMP1 antibody (A), a-STEVOR antibody (B), a -SBP 1 antibody (C) and a -REX 1 antibody (D). Scale bar: 1 μm; same size of ROI in each image. In CS2ΔPTP1 the architecture of MC was disrupted showing large aggregated globular structures. SBP1 and REX1 were located on these structures reflecting the results obtained by IFA.
Rug M, Cyrklaff M, Mikkonen A, Lemgruber L, Kuelzer S, Sanchez CP, Thompson J, Hanssen E, O'Neill M, Langer C, Lanzer M, Frischknecht F, Maier AG, Cowman AF. Export of virulence proteins by malaria-infected erythrocytes involves remodelling of host actin cytoskeleton. Blood. 2014 Aug 19 [Epub ahead of print]
Other associated proteins
|PF3D7_0501300||skeleton-binding protein 1|
|PF3D7_0935900||ring-exported protein 1|