Subcellular localization of the SR proteins in the blood stage. Mixed asexual blood stage cultures containing trophozoites (TZ) and schizonts (SZ) cultures were fixed with paraformaldehyde and prepared for IFA, using mouse antisera against PfSRSF12, PfSFRS4 and PfSF-1 (green). The parasite nuclei were highlighted by Hoechst staining (blue). The parasites were labelled with rabbit antisera against PfMSP-1. Bar, 5 mm. IFAs detected the three SR proteins in the trophozoite nucleus (top rows). An additional minor labelling was observed in the nuclei of the schizont stages (center rows).

Kern S, Agarwal S, Huber K, Gehring AP, Strödke B, Wirth CC, Brügl T, Abodo LO, Dandekar T, Doerig C, Fischer R, Tobin AB, Alam MM, Bracher F, Pradel G. Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission. PLoS One. 2014 Sep 4;9(9):e105732.