PF3D7_0628100 hect domain-containing protein 1, putative

HECT ubiquitin-protein ligase (PfUT) localizes to the ER/Golgi complex. Right panels: Subcellular localization of PfUT by immunoelectron microscopy. The upper panel shows a representative micrograph of a P. falciparum-infected erythrocyte preserved by high-pressure freezing and freeze-substitution, and immunolabelled with a rabbit antiserum specific to the N-terminal domain of PfUT coupled to 10 nm protein A colloidal gold. The lower panel shows the surface rendered view of the micrograph, with red dots representing gold grains. Left: Magnification of boxed section in micrograph. Arrowheads point towards gold label. n, nucleus; fv, food vacuole. Scale bar in D and E, 500 nm. Quantitative immunoelectron microscopy confirmed a predominant localization of PfUT at the ER/Golgi complex.

Sanchez CP, Liu CH, Mayer S, Nurhasanah A, Cyrklaff M, Mu J, Ferdig MT, Stein WD, Lanzer M. A HECT ubiquitin-protein ligase as a novel candidate gene for altered quinine and quinidine responses in Plasmodium falciparum. PLoS Genet. 2014 10(5):e1004382.