PF3D7_0206800 merozoite surface protein 2

GPI-anchored MSP2 is carried into invaded RBCs and rapidly degraded. (A) Invading merozoites were labeled with the tight junction marker PfRON4

(green) and colabeled with antibodies to different regions of MSP2 (red). All regions of MSP2 were carried through the tight junction into the RBC, with labeling being visible on both sides of PfRON4 at the tight junction. (B) Newly invaded rings (10 min postinvasion) were labeled with antibodies to MSP1-19 (green) and antibodies to different regions of MSP2 (red). MSP2 was rapidly degraded postinvasion, with little or no labeling being visible in rings. Images are single slices from deconvoluted stacks. Representative images of antibodies to the different regions of MSP2; other antibodies tested showed the same labeling patterns. The MSP2 allele of the parasite strain used in each row of images is indicated as 3D7 parasite or FC27 parasite (D10 parasite strain). Antibody dilutions used were 1:500 for secondary antibodies, 1:250 for 8G10, 1:100 for PfRON4, and 1:50 for N-terminal purified antibodies, 9D11, and

9G8.

Boyle MJ, Langer C, Chan JA, Hodder AN, Coppel RL, Anders RF, Beeson JG. Sequential processing of merozoite surface proteins during and after erythrocyte invasion by Plasmodium falciparum. Infect Immun. 2014 82(3):924-36.

Other associated proteins

PFID Formal Annotation
PF3D7_1116000 rhoptry neck protein 4
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