PFB0080c is expressed on the IRBC surface of C4S-selected P. falciparum. A-D, Immunofluorescence analysis of IRBCs was performed using anti-PFB0080c antiserum followed by either FITC-conjugated goat anti-mouse IgG (A-C) or phycoerythrin-conjugated goat antimouse IgG (D) secondary antibody. A and B, Permeabilized C4S-adherent (3D7-CSA) IRBCs (A) and non-selected (3D7-NS) IRBCs (B). C and D, Live 3D7-CSA IRBCs. PFB0080c is strongly expressed on the surface of C4S adherent IRBCs, but not in 3D7-NS IRBCs.

Goel S, Muthusamy A, Miao J, Cui L, Salanti A, Winzeler EA, Gowda DC. Targeted disruption of a ring-infected erythrocyte surface antigen (RESA)-like export protein gene in Plasmodium falciparum confers stable chondroitin 4-sulfate cytodherence capacity. J Biol Chem. 2014 Oct 23. [Epub ahead of print]