PFB0080c-knockout P. falciparum express VAR2CSA on the surface of infected erythrocytes. A and B, Immuno-fluorescence analysis of VAR2CSA in live ΔPFB0080c IRBCs was performed using rabbit anti-VAR2CSA antiserum followed by biotin-conjugated sheep antirabbit IgG secondary antibody and phycoerythrin-conjugated streptavidin. Shown are the immunofluorescent and light micrographs of parental 3D7-CSA parasites (A) and ΔPFB0080c parasites (B); both parasite types expressed VAR2CSA protein.

Goel S, Muthusamy A, Miao J, Cui L, Salanti A, Winzeler EA, Gowda DC. Targeted disruption of a ring-infected erythrocyte surface antigen (RESA)-like export protein gene in Plasmodium falciparum confers stable chondroitin 4-sulfate cytodherence capacity. J Biol Chem. 2014 Oct 23. [Epub ahead of print]