Expression and localization of Atg8 in asexual erythrocytic stages. The indicated stages of P. falciparum were evaluated for the presence of Atg8 by IFA using anti-Atg8 antibodies. The images of each indicated stage show the presence of Atg8 specific signal (Atg8), nucleic acid staining (DAPI), the parasite and the erythrocyte boundaries (Bright field), and the merged of all three images (Merge). The Atg8 signal is present throughout the parasite in all the stages shown, and appears to be associated with punctate structures, most likely autophagosomes. The scale bar shown is identical for this and all the figures containing IFA images.

Navale R, Atul, Allanki AD, Sijwali PS. Characterization of the Autophagy Marker Protein Atg8 Reveals Atypical Features of Autophagy in Plasmodium falciparum. PLoS One. 2014 Nov 26;9(11):e113220.