Mutational analysis of the minimal sequence requirements for IMC association of PfISP1. AB. Expression of the first 11 (11PfSP1-GFP, A) or 10 (10PfISP1-GFP, B) amino acids are sufficient to direct GFP to the IMC (A1-B1). C. Substitution

of D11 with lysine (11PfISP1D11K-GFP, C) abolishes IMC targeting (C1) and leads to peripheral localization. The zoom highlights its additional association with the food vacuole membrane consistent with plasma membrane association. C2. Co-localization with the IMC marker GAPM2. Nascent IMC (anti-GAPM2, red) is clearly distinguishable from the peripheral associated 11PfISP1D11K-GFP in early stages (T1) and congruent in late stages (T3).

Wetzel J, Herrmann S, Swapna LS, Prusty D, Peter AT, Kono M, Saini S, Nellimarla S, Wong TW, Wilcke L, Ramsay O, Cabrera A, Biller L, Heincke D, Mossman K, Spielmann T, Ungermann C, Parkinson J, Gilberger TW. The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite. J Biol Chem. 2014 Nov 25.