IFA images of schizonts of the indicated transgenic and parental 3D7 clones, probed with a polyclonal rabbit antiserum raised against full-length rSERA5wt (green) or the anti-MSP1 monoclonal antibody (mAb) 89.1 (red). The merged images include the fluorescence signal of the nuclear stain DAPI. No morphological differences were evident between the parental and transgenic parasites. Scale bar, 1 mm. IFA analysis showed normal expression and distribution of SERA5 in all clones.

Stallmach R, Kavishwar M, Withers-Martinez C, Hackett F, Collins CR, Howell SA, Yeoh S, Knuepfer E, Atid AJ, Holder AA, Blackman MJ. Plasmodium falciparum SERA5 plays a non-enzymatic role in the malarial asexual blood-stage lifecycle. Mol Microbiol. 2015 Jan 20. [Epub ahead of print]