PF3D7_0917900 PfHsp70-2

Pfμ1 is closely associated with the Golgi and ER in early trophozoite stages. Transgenic parasites expressing Pfμ1–GFP protein at trophozoite stages were immunostainedwith anti-PfGRASP (A), anti-PfBiP (B) and anti-PfRab7 (C) antibodies. The parasite nuclei were stained with DAPI and slides were visualized by confocal laser scanning microscopy. Scale bars denote 5 μm. Pfμ1 partially co-localized with PfGRASP and PfBip. the ER (which is contiguous with the nuclear envelope) and Golgi in developing merozoites are very closely juxtaposed, and dense vesicular traffic occurs between these two compartments, as well as between nascent rhoptries. No overlap between Pfμ1–GFP and Rab7.This suggests that these proteins do not co-localize, and hence that Rab7 is not involved in trafficking at this stage (3C). These results thus suggest the presence of Pfμ1 in the Golgi–ER network during the early stages of intra-erythrocytic development.

Kaderi Kibria KM, Rawat K, Klinger CM, Datta G, Panchal M, Singh S, Iyer GR, Kaur I, Sharma V, Dacks JB, Mohmmed A, Malhotra P. A role for adaptor protein complex 1 in protein targeting to rhoptry organelles in Plasmodium falciparum. Biochim Biophys Acta. 2015 1853(3):699-710.

Other associated proteins

PFID Formal Annotation
PF3D7_0903200 ras-related protein RAB7
PF3D7_1017300 golgi re-assembly stacking protein 2
PF3D7_1311400 AP-1 complex subunit mu-1