Subcellular location of PfAK2/GFP and the non-myristoylated G2A variant, as determined by live fluorescence microscopy (A) The AK2/GFP and the AK2G2A/GFP fusion proteins were expressed using the CRT promoter from an episomal plasmid (constructs indicated above the images). AK2/GFP is located at the periphery of the intracellular parasite as judged by epifluorescence microscopy, and is associated with one or two protuberances towards the host cell cytoplasm present on each parasite (indicated by white arrow). In contrast, the AK2G2A/GFP chimera is located within the parasite cytosol. The infected cell was visualised by differential interference contrast (DIC), intrinsic fluorescence of the GFP identified the location of the AK2/GFP fusion protein, and parasite nuclei were detected by Hoechst staining. Overlay: green (GFP), blue (DNA). Scale bar-3 μm.
Thavayogarajah T, Gangopadhyay P, Rahlfs S, Becker K, Lingelbach K, Przyborski JM, Holder AA. Alternative Protein Secretion in the Malaria Parasite Plasmodium falciparum. PLoS One. 2015 Apr 24;10(4):e0125191.
Other associated proteins
|PF3D7_1020900||ADP-ribosylation factor 1|