Effect of ACF on replication foci formation. Immunofluorescence assay to show pattern of replication foci (PCNA foci) formation during parasite developmental stages in untreated, Acriflavine (100 nM) and hydroxyurea (70 μg/mL) treated parasites. DAPI was used for nuclear staining. Panel III shows the merged images of DAPI (I) and PCNA (II) signals whereas panel IV represents the merged images of PCNA signal, DAPI and DIC images. Immunofluorescence results indicate the presence of diffused signals of PCNA in Acriflavine as well as hydroxyurea- treated parasites compared to the distinct nuclear foci found in untreated parasites.
Dana S, Prusty D, Dhayal D, Gupta MK, Dar A, Sen S, Mukhopadhyay P, Adak T, Dhar SK. Potent antimalarial activity of acriflavine in vitro and in vivo. ACS Chem Biol. 2014 9(10):2366-73.