Localization of falcipain 1 (FP1) in merozoites by immunofluorescence imaging. Samples of merozoites were smeared and fixed in 1% paraformaldehyde. The slides were incubated with an antibody to falcipain 1 or with antibodies specific to the rhoptry (RopH2) and microneme (EBA-175) proteins. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI). The lack of colocalization indicates that falcipain 1 is found in compartments at the apical end of the merozoite that are distinct from the rhoptries and micronemes.
Greenbaum DC, Baruch A, Grainger M, Bozdech Z, Medzihradszky KF, Engel J, DeRisi J, Holder AA, Bogyo M. A role for the protease falcipain 1 in host cell invasion by the human malaria parasite. Science. 2002 298(5600):2002-6.
PubMed Article: A role for the protease falcipain 1 in host cell invasion by the human malaria parasite
Other associated proteins
|PF3D7_0929400||high molecular weight rhoptry protein 2|
|PF3D7_1458000||cysteine proteinase falcipain 1|