Live transgenic cell lines reveal fluorescent chimera (N-terminal 63 amino acids of KAHRP including the signal sequence (red) and PEXEL (blue) attached to GFP; 2: N-terminal 69 amino acids of KAHRP attached to GFP; 3: N-terminal 63 amino acids of KAHRP plus seven alanine residues which act as a spacer between the PEXEL) expression in different parts of P. falciparum-infected erythrocytes. Shown as labelled are K63-GFP, K69-GFP and K63A7. Shown for each panel block are ring, trophozoite (troph) and schizont stages with GFP fluorescence (first image), DAPI nuclear stain (second image), phase contrast image (third image) and merge of the GFP and phase contrast image (fourth image). Strong GFP fluorescence is typically seen in trophozoites and schizonts for all GFP chimeras that are located in or transit the parasitophorous vacuole.
Knuepfer E, Rug M, Cowman AF. Function of the plasmodium export element can be blocked by green fluorescent protein. Mol Biochem Parasitol. 2005 142(2):258-62.