CDPK1 localizes to the plasma membrane of merozoites. A, an anti-CDPK1 antibody detects CDPK1 at the parasite periphery in schizonts (panel i) and free merozoites (panel ii). The second panel shows a merged image of nuclear staining with 4’,6’-diamino-2-phenylindole (blue) and the bright field image, and the third panel shows the bright field image. B, panel i, CDPK1 colocalizes with MTIP around the periphery of merozoites but is also found around the residual body, marked with an arrowhead, from which MTIP is absent. In young (panel ii) and segmented (panel iii) schizonts, CDPK1 colocalizes with the plasma membrane marker MSP1. Both proteins are also detected around the residual body, marked with an arrowhead. CDPK1 does not colocalize with the food vacuole marker PfCRT in either immature (panel iv) or mature (panel v) schizonts. The third panels in all of the images show the merged images of the antibody staining, beside which is a merged image of the bright field image and the nuclei of the parasites stained with 4’,6’-diamino-2-phenylindole (blue), followed by the bright field image alone. C, both CDPK1 (red) and MSP1 (green) can be detected on residual bodies that are released upon schizont rupture. The merged images of the antibody staining are shown in the third panels, followed by the bright field image. In all cases the white scale bar in the first panel of each set of images represents 1 mm.
Green JL, Rees-Channer RR, Howell SA, Martin SR, Knuepfer E, Taylor HM, Grainger M, Holder AA. The motor complex of Plasmodium falciparum: phosphorylation by a calcium-dependent protein kinase. J Biol Chem. 2008 7;283(45):30980-9. PMID:
Other associated proteins
|PF3D7_0217500||Calcium-dependent protein kinases; CDPK1|
|PF3D7_0709000||chloroquine resistance transporter|
|PF3D7_0930300||merozoite surface protein 1|