MyoB subcellular location in asexual blood and mosquito stages. A. Microscopic analysis of live P. falciparum asexual blood stage parasites expressing GFP tagged MyoB. Shown are blood stage parasites of increasing maturity from early schizogony (two to four nuclei; 30 hours post invasion [hpi]) through to mature segmenter forms (44 hpi) and free merozoites analysed by fluorescence microscopy. GFP was detected by green fluorescence and the nuclei (blue) were labelled with Hoechst dye prior to microscopic analysis. GFP fluorescence was not detected in trophozoites (not shown) and early schizont stages, and was first apparent in mature multinucleate schizonts as a number of single dots (40 hpi). Following cytokinesis a single dot was present associated with each nucleus at what appeared to be the apical end of the cell. The images, merged with the differential interference contrast (DIC) image, are shown in the right panel. Scale bar: 2 μm.
Yusuf NA, Green JL, Wall RJ, Knuepfer E, Moon RW, Schulte-Huxel C, Stanway RR, Martin SR, Howell SA, Douse CH, Cota E, Tate EW, Tewari R, Holder AA. The Plasmodium Class XIV Myosin, MyoB has a Distinct Subcellular Location in Invasive and Motile Stages of the Malaria Parasite, and an Unusual Light Chain. J Biol Chem. 2015 Mar 23.