Loss of food vacuolar integrity upon fosmidomycin and wortmannin treatment. Shown is the confocal fluorescence microscopic localization of a plasmepsin II-GFP (P2-GFP) construct (left) or live fluorescence imaging of Lysotracker Red-stained malaria parasites (right). Control parasites (A) are compared to parasites treated for 24 h with either fosmidomycin (B), fosmidomycin plus geranylgeraniol (C), or wortmannin (D). Images are representative of at least three independent biological experiments. Visualization of PMIIGFP in FSM- and wortmannin-treated parasites required higher detector gain levels, resulting in increased observed erythrocyte autofluorescence. Lower panel: Apicoplast and parasitophorous vacuolar targeting in fosmidomycin- and wortmannin-treated parasites. Shown is live-cell fluorescence of malaria parasites that express either the leader sequence (ACPL-GFP) (A) or signal sequence (ACPs-GFP) (B) from P. falciparum acyl carrier protein, fused to GFP, which traffic to the apicoplast or parasitophorous vacuole, respectively. Untreated parasites (control) are compared to fosmidomycin (FSM)- and wortmannin (wort)-treated parasites. Images are representative of at least three independent biological experiments.
Howe R, Kelly M, Jimah J, Hodge D, Odom AR. Isoprenoid biosynthesis inhibition disrupts Rab5 localization and food vacuolar integrity in Plasmodium falciparum. Eukaryot Cell. 2013 Feb;12(2):215-23.
Other associated proteins